Expressing:Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.
Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.
Article Title: Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer
Article Snippet: Five human gastric carcinoma cell lines (MGC-803, SGC-7901, AGS, BGC-823, MKN-45) and a normal human gastric mucosal epithelial cell line (GES-1) were purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).. Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .
Immunohistochemical staining:Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.
Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.
Article Title: Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer
Article Snippet: Five human gastric carcinoma cell lines (MGC-803, SGC-7901, AGS, BGC-823, MKN-45) and a normal human gastric mucosal epithelial cell line (GES-1) were purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).. Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .
Staining:Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.
Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.
Article Title: Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer
Article Snippet: Five human gastric carcinoma cell lines (MGC-803, SGC-7901, AGS, BGC-823, MKN-45) and a normal human gastric mucosal epithelial cell line (GES-1) were purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).. Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .
Western Blot:Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.
Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.
Article Title: Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer
Article Snippet: Five human gastric carcinoma cell lines (MGC-803, SGC-7901, AGS, BGC-823, MKN-45) and a normal human gastric mucosal epithelial cell line (GES-1) were purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).. Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .
Control:Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.
Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.
Article Title: Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer
Article Snippet: Five human gastric carcinoma cell lines (MGC-803, SGC-7901, AGS, BGC-823, MKN-45) and a normal human gastric mucosal epithelial cell line (GES-1) were purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).. Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .
Whisker Assay:Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.
Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.
Article Title: Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer
Article Snippet: Five human gastric carcinoma cell lines (MGC-803, SGC-7901, AGS, BGC-823, MKN-45) and a normal human gastric mucosal epithelial cell line (GES-1) were purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).. Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .
Comparison:Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% (v/v) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 ◦C with 5% CO2, respectively.
Article Title: Development of a Graphene Oxide-Based Aptamer Nanoarray for Improved Neutralization and Protection Effects Against Ricin
Article Snippet: Normal human alveolar epithelial cell line (BEAS-2B), human gastric mucosal epithelial cell line (GES-1), and human liver cell line (HL7702) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).. Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.Cells were incubated in Dulbecco’s modified eagle medium (DMEM) supplemental with 10% ( v / v ) fetal bovine serum (FBS, Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) in a humidified incubator at 37 °C with 5% CO 2 , respectively.
Article Title: Leucine-rich repeat-containing G-protein-coupled receptor 5 is associated with invasion, metastasis, and could be a potential therapeutic target in human gastric cancer
Article Snippet: Five human gastric carcinoma cell lines (MGC-803, SGC-7901, AGS, BGC-823, MKN-45) and a normal human gastric mucosal epithelial cell line (GES-1) were purchased from the American Tissue Culture Collection (ATCC, Manassas, VA, USA).. Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .Cells were cultured in RPMI 1640 medium (GIBCO-BRL, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; GIBCO-BRL), 100 U ml −1 penicillin, and 100 μ g ml −1 streptomycin at 37 °C/5% CO 2 .
|
ATCC is a verified supplier 
ATCC manufactures this product 